Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Top 10 Hub Genes With Higher Degree Scores.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: Protein Binding, Binding Assay, Activity Assay, Transduction, Ubiquitin Proteomics, Phospho-proteomics

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Correlation between methylation levels and cancer-testis (CT) gene expression. Promoter methylation and gene expression data from The Cancer Genome Atlas (TCGA) were visualized using cBioPortal to demonstrate the correlation among selected hub CT genes, and significant negative relationship was identified in: (A) ARUKA, (B) ASPM, (C) BUB1, (D) BUB1B, (E) CCNB2, (F) NUF2, (G) PBK, (H) TPX2.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: Methylation, Gene Expression

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Prognostic values of 9 cancer-testis (CT) genes identified by overall survival analysis using Kaplan-Meier curve from GEPIA: (A) ASPM, (B) ARUKA, (C) BUB1, (D) BUB1B, (E) CDC45, (F) DLGAP5, (G) NUF2, (H) PBK, (I) TPX2.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: BUB1B might be a potential target for immunotherapy in hepatocellular carcinoma (HCC). (A) Association of the BUB1B expression and CD8 + T-cell infiltration in HCC by TISIDB (r = −0.129, P = .013). (B) BUB1B expression level is negatively associated with CD8 + T-cell infiltration level validated in our 72 patients, tested by Spearman test (r = −0.489, P < .001). (C) Representative immunohistochemical staining of BUB1B, β-catenin, and CD8 in HCC. Negative, weak positive, moderate positive, and strong positive expression of BUB1B were shown, respectively. (D) Association of the BUB1B messenger RNA (mRNA) expression and β-catenin mRNA in HCC by GEPIA (r = 0.46, P < .001). (E) BUB1B expression level is positively associated with β-catenin expression level validated in our 72 patients, tested by Spearman test (r = −0.588, P < .001). (F) Overall survival (OS) curves of patients with HCC according to cancer expressed BUB1B levels ( P < .001). (G) Disease-free survival (DFS) curves of patients with HCC according to cancer expressed BUB1B levels ( P = .008).

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: Expressing, Immunohistochemical staining, Staining

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Clinical and Pathological Features of the 72 Patients.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Univariate and Multivariate Analyses of Clinicopathologic Factors in Patients With HCC With Respect to OS.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques:

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Univariate and Multivariate Analyses of Clinicopathologic Factors in Patients With HCC With Respect to DFS.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: Pan-cancer analysis of BUB1B expression. ( A ) Differential expression of BUB1B between tumor and normal tissues in pan-cancer analysis. ( B , C ) Expression of BUB1B in various cancer cell lines and tissues. ( D,E ) Cellular localization of BUB1B from U-251MG and U2OS. ** p < 0.01, *** p < 0.001.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Expressing, Quantitative Proteomics

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: BUB1B expression correlates with overall survival time (OS). GEPIA2 analyses of the association between BUB1B expression and OS in ( A ) ACC, ( B ) KIRC, ( C ) KIRP, ( D ) LGG, ( E ) LIHC, ( F ) LUAD, ( G ) MESO, ( H ) PAAD, ( I ) SARC, and ( J ) THYM.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: Correlation between BUB1B expression and mutations in various cancer types. ( A ) Landscape of BUB1B mutation in 32 cancer types, ( B ) the subtypes and distributions of BUB1B somatic mutations, ( C , D ) Spearman correlation analysis for TMB, MSI, and BUB1B gene expression. In the figure, the horizontal axis represents the correlation coefficient between the genes and TMB, and the vertical axis represents the different tumors. The size of the dots in the figure represents the correlation coefficient, and the different colors represent the significance of the p value. The bluer the color in the diagram, the smaller the p value.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Expressing, Mutagenesis, Gene Expression

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: The association between BUB1B expression and immune cell infiltration. ( A , B ) BUB1B expression is positively associated with MDSC infiltration in pan-cancer. ( C , D ) BUB1B expression is positively negative with NKT cell infiltration in pan-cancer. ( E – H ) BUB1B expression is negative associated with the infiltration level of B cells, macrophage cells, CD4+ T cells, and CD8+ T cells.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: The enrichment analysis of BUB1B co-expression genes in LUAD. ( A ) The BUB1B co-expression genes in LUAD. ( B , C ) The top 50 genes positively and negatively correlated to BUB1B. ( D , E ) GO and KEGG analysis of BUB1B co-expression genes in the LUAD cohort.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Expressing

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: Function of BUB1B in LUAD determined using the CancerSEA database. ( A ) Analysis from the CancerSEA database at single-cell resolution indicated that BUB1B was primarily involved in cell cycle, proliferation, DNA damage, DNA repair, invasion, inflammation, quiescence. ( B , C ) Functional relevance in LUAD, BUB1B expression was significantly positively correlated with cell cycle, proliferation, DNA damage, DNA repair, invasion, and was negatively correlated with inflammation, quiescence. The experiments were repeated three times. (* p < 0.05, ** p < 0.01, *** p < 0.001).

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Functional Assay, Expressing

Journal: International Journal of Molecular Sciences

Article Title: A Comprehensive Analysis Revealing BUB1B as a Potential Prognostic and Immunological Biomarker in Lung Adenocarcinoma

doi: 10.3390/ijms26052061

Figure Lengend Snippet: Cellular functions of BUB1B. ( A , B ) Western blot and RT-qPCR detection after siRNA-mediated knockdown of BUB1B in A549 cells. ( C ) CCK-8 assay results showing the decrease in the viability of A549 cells upon the knockdown of BUB1B. ( D , E ) Wound healing test. ( F , G ) Transwell assay results showing the decrease in the migration in A549 cells upon the knockdown of BUB1B. ( H , I ) Western blot results showing the decrease in the EMT progression in A549 cells upon the knockdown of BUB1B. All experiments were repeated three times with three replicates for each repeat. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: The information for antibodies was as follows: BUB1B (Boster, Wuhan, China), vimentin (CST, Danvers, MA, USA), E-cadherin (CST, MA, USA), N-cadherin (CST, MA, USA), and GAPDH (CST, MA, USA).

Techniques: Western Blot, Quantitative RT-PCR, Knockdown, CCK-8 Assay, Transwell Assay, Migration

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Top 10 Hub Genes With Higher Degree Scores.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: Protein Binding, Binding Assay, Activity Assay, Transduction, Ubiquitin Proteomics, Phospho-proteomics

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Correlation between methylation levels and cancer-testis (CT) gene expression. Promoter methylation and gene expression data from The Cancer Genome Atlas (TCGA) were visualized using cBioPortal to demonstrate the correlation among selected hub CT genes, and significant negative relationship was identified in: (A) ARUKA, (B) ASPM, (C) BUB1, (D) BUB1B, (E) CCNB2, (F) NUF2, (G) PBK, (H) TPX2.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: Methylation, Gene Expression

Journal: Technology in Cancer Research & Treatment

Article Title: Cancer-Testis Gene Expression in Hepatocellular Carcinoma: Identification of Prognostic Markers and Potential Targets for Immunotherapy

doi: 10.1177/1533033820944274

Figure Lengend Snippet: Prognostic values of 9 cancer-testis (CT) genes identified by overall survival analysis using Kaplan-Meier curve from GEPIA: (A) ASPM, (B) ARUKA, (C) BUB1, (D) BUB1B, (E) CDC45, (F) DLGAP5, (G) NUF2, (H) PBK, (I) TPX2.

Article Snippet: Primary antibodies used for immunohistochemistry (IHC) of paraffin-embedded 72 HCC tissues and their paired noncancerous tissues included rabbit polyclonal anti-BUB1B antibody (1:200, Proteintech, Cat No. 11504-2-AP), rabbit polyclonal anti-ASPM antibody (1:100, Proteintech, Cat No. 26223-1-AP), rabbit polyclonal anti-beta catenin antibody (1:2000, Proteintech, Cat No. 51067-2-AP), and mouse monoclonal anti-CD8 antibody (1:200, Abcam, Cat No. ab17147).

Techniques: